General instructions for use.
Monoclonal and polyclonal antibodies are used in a large variety of technical procedures. Methods to be selected are dependent on the specific demands and materials available. The methods given below may offer a basis, which can be adapted to local demands and available materials. See for further technical help: Current Protocols in Immunology, ed J.E. Coligan et al., J. Wiley & sons, New York 1991, ISBN 0-471-52276-7.
All antibody protocols are available by clicking on the link:
- ABC Technique using Cultured Cells Grown on Glass Cover Slips
- ABC Technique using Monoclonal Antibodies on Frozen Sections
- ABC Technique using Monoclonal Antibodies on Paraffin Sections
- ABC Technique using Polyclonal Antibodies on Frozen Sections
- ABC Technique using Polyclonal Antibodies on Paraffin Sections
- Antigen Unmasking Technique using EDTA
- Antigen Unmasking Technique using Sodium Citrate Buffer
- Description of Methods for the Use of Monosan Antibodies in Direct Flow Cytometry
- Description of Methods for the Use of Monosan Antibodies in Indirect Flow Cytometry
- Method for Use of Antibodies in Western Blotting
- Storage of Antibodies in Soluble Form
- Storage of Lyophilised Antibodies
- Trypsin Digestion of FFPE Tissues Prior to IHC
- Zamboni’s-fixed Frozen Sections in IHC